摘要
介绍了一种简便易行的制备蔗果三糖标准品的方法。研究得出曲霉６．２２２胞内、胞外酶均具有活性，最佳酶反应条件为：ｐＨ６．０，温度５０℃，时间６０ｍｉｎ。最佳初始蔗糖浓度为２５０ｇ／Ｌ。由曲霉６．２２２发酵制备的酶溶液稳定性良好。
It is available to prepare standard 1kestose easily. The intracellular and extracellular activities of the strain 6.222 (isolated from soil and identified as moulds) were investigated. The optimum enzymatic reaction conditions of it were as follows: pH6.0, 50, 60min. The optimal initial concentration of sucrose in enzymatic reaction is 250g/L. The stability of enzymatic solution is excellent.
出处
《华东理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
1999年第4期371-373,381,共4页
Journal of East China University of Science and Technology
基金
上海市自然科学基金
关键词
蔗果三糖
果糖低聚糖
微生物酶法
制备
fructooligosaccharides
1kestose
enzymatic activity
enzymatic reaction
