中国花生小核心种质SSR遗传多样性

Genetic diversity of peanut mini core collection detected by SSR markers

从206对SSR引物中筛选26对引物对我国花生全套小核心种质298份资源进行了遗传多样性分析,相似系数为0.51～0.99,其中种质zhh1497与zhh1398遗传差异最大。检测到3个在不同植物学类型中特异表达的标记带型,包括普通型1个(2A06/440),龙生型1个(2A06/440),珍珠豆型1个(PM443/270),多粒型2个(PM443/270,9E08/500)。分析结果表明,多粒型花生的多态性信息量和遗传多样性指数均最大,平均相似系数最小,其次是普通型花生,龙生型和中间型花生的多态性信息量和遗传多样性指数均较小,平均相似系数较大。在我国花生小核心种质中,国外引入资源的多态性信息量和多样性指数均高于我国本土资源的对应值。在我国本土花生资源中,长江流域和南方地区资源的多态性信息量和遗传多样性指数均高于其他地区。

A set of mini core collection comprised of 298 accessions was employed for SSR analysis.Twenty six polymorphic SSR markers were screened from a total of 206 primer pairs.The similarity coefficients between genotype pairs among the 298 accessions were 0.51 to 0.99.The genetic difference between the genotypes of zhh1497 and zhh1398 was the highest with similarity coefficient of 0.51 among these accessions.Three special SSR markers in botanical types were detected including 1 marker （2A06/440） in the type of hypogaea,1（2A06/440） in hirsuta,1 （PM443/270） in vulgaris and 2 （PM443/270,9E08/500） in fastigiata.Molecular polymorphism detected by SSR indicated that genetic diversity index were the highest in accessions of fastigiata,followed by hypogaea,vulgaris,hirsuta and then by the intermediate type.Among the Chinese mini core collection,both polymorphic information content and genetic diversity index were higher in introduced foreign accessions than the corresponding traits in the native accessions.In terms of Chinese native resource,the polymorphic information content and genetic diversity index were higher in accessions from Yangtze River valley and region of southern China than the corresponding traits in accessions from other regions of China.