提莫菲维小麦高分子量谷蛋白亚基遗传多样性分析

Genetic diversity of High Molecular Weight Glutenin Subunits in Triticum timopheevii Zhuk.

为了给普通小麦的品质改良提供基础材料,并了解提莫菲维小麦的HMW-GS组成情况,利用SDS-PAGE技术对43份提莫菲维小麦高分子量谷蛋白亚基遗传多样性进行了分析,分别发现2、3、2和1种Ax、Ay、Gx和Gy亚基的等位变异类型和5种组合类型[Ax2*,Gx(A);Ax2*+Ay(C),Gx(B)+Gy;Ax1+Ay(B),Gx(B)+Gy;Ax1+Ay(A),Gx(A);Ay(A),Gx(B)]。其中Ax2*,Gx(A)的分布频率最高,达81.40%。在43份材料中,有12份材料(27.90%)的Ay亚基表达,Gx亚基几乎都表达,而只有部分Gy亚基可表达,但频率非常低,仅为6.98%。结果表明提莫菲维小麦Glu-A1的遗传多样性高于Glu-G1。文中对检测到的各种亚基的利用方式进行了讨论。

In order to provide basic materials for the quality improvement and detect the HMW-GS components of Triticum timopheevii,43 T.timopheevii were evaluated by means of SDS-PAGE.There were 2,3,1 and 2 different Ax,Ay,Gx and Gy subunits,and 5 HMW-GS combinations [Ax2＊,Gx（A）;Ax 2＊＋Ay（C）,Gx（B）＋Gy;Ax1＋Ay（B）,Gx（B）＋Gy;Ax1＋Ay（A）,Gx（A）;Ay（A）,Gx（B）],Among which,combination Ax2＊,Gx（A）,accounting for 81.40% of the total T.timopheevii accessions,presented most frequently.The expressed Ay and Gy subunits were detected in T.timopheevii accessions,amounting to 27.90% and 6.98%,respectively.These results indicated that the Glu-A1 had higher genetic diversity than the Glu-G1.The utilization strategy of these subunits was discussed.