LKB1基因沉默对乳腺癌细胞生物学行为的影响

Effect of siRNA-mediated LKB1 gene silence on the biological behavior of breast carcinoma cells

目的研究LKB1基因表达沉默对乳腺癌细胞生物学行为的影响。方法以RNA干扰技术建立LKB1表达抑制的细胞模型,RT-PCR和Western印迹法检测干扰LKB1蛋白表达的效果;通过绘制细胞生长曲线检测LKB1基因沉默乳腺癌细胞的增殖变化;流式细胞技术分析LKB1基因沉默后乳腺癌细胞周期的变化及其凋亡和增殖情况。结果成功建立了LKB1基因沉默乳腺癌细胞模型,稳定表达LKB1-siRNA细胞的LKB1蛋白表达明显被抑制,LKB1基因沉默后乳腺癌细胞增殖明显加快。结论 LKB1基因的下调表达对乳腺癌细胞部分恶性生物学行为的发生有一定的促进作用。

Objective To study the effect of the small interfering RNA（siRNA）-mediated LKB1 gene silencing on the biological behavior of breast carcinoma cells.Methods RNA interference technique was used to silence LKB1 gene in breast carcinoma cell,and the expression of LKB1 protein were subsequently detected by Western blotting and RT-PCR.The cell proliferation was then assessed by observing the growth curve of the cells,and cell cycle and apoptosis were assayed by flow cytometry.Results LKB1 siRNA resulted in remarkable suppression of LKB1 expression in the breast carcinoma cells.LKB1 gene silencing resulted in accelerating cell proliferation,but cell apoptosis underwent no significant changes.Conclusion Down-regulation of LKB1 gene expression can stimulate malignant biological behavior of breast carcinoma cells.