三氧化二砷对人肺癌A549细胞凋亡及耐药基因表达的影响

Effect and mechanism of arsenic trioxide on LRP mRNA expression in human lung adenocarcinoma cells in vitro

目的研究三氧化二砷（As2O3）对人肺癌细胞株的诱导凋亡作用及对耐药基因LRP表达的影响。方法应用人肺腺癌A549细胞株，运用体外细胞培养法、MTT法、流式细胞术检测As2O3对人肺癌细胞株抑制及诱导凋亡作用；用逆转录-聚合酶链反应（RT-PCR）方法检测LRP mRNA的表达。结果As2O3对人肺癌A549细胞具有抑制作用，其抑制率呈时间-剂量依赖关系。不同浓度的As2O3均可诱导凋亡。1．0μmol／L、3．0μmol／L的As2O3可下调LRPmRNA的表达。结论As2O3具有抑制肿瘤细胞生长及诱导细胞凋亡作用，其机制与下调LRPmRNA表达有密切关系。

Objective To study the mechanism of arsenic on human lung adenocarcinoma cells. Methods MTT, FCM and RT-PCR tests were used to investigate apoptosis levels induced by arsenic trioxide and the effect on LRP mRNA expression. Results 1.0μmol/L and 3.0μmol/L arsenic trioxide can decrease LRP mRNA expression. Conclusion Low concentration As2O3 may improve apoptosis of lung cancer by decreasing LRP mRNA expression. As2O3 may be used to treat lung cancer but the results are not better than cisplatin.