神经生长因子及其高亲和力受体在慢性阻塞性肺疾病患者细支气管上皮细胞中的表达

A study on expressions of nerve growth factor and its receptor tyrosine kinase receptor A in bronchiolar epithelial cells of chronic obstructive pulmonary disease patients

目的 探讨神经生长因子和酪氨酸激酶A在COPD患者细支气管上皮细胞中的表达.方法 选取同济医学院附属同济医院胸外科2008年1-6月因肺肿瘤行肺叶或全肺切除术患者31例为研究对象,分为吸烟合并COPD组11例,均为男性,年龄42～79岁,平均（61±11）岁;吸烟无COPD组11例,均为男性,年龄45～67岁,平均（56±6）岁;对照组9例,其中女7例,男2例,年龄38～68岁,平均（52±10）岁.取石蜡包埋的非肿瘤病灶病理组织切片,采用免疫组织化学SP法检测神经生长因子和酪氨酸激酶A在细支气管上皮细胞中的表达水平.组间差异采用方差分析及q检验,采用Person法进行相关性分析.结果 吸烟合并COPD组细支气管上皮细胞中神经生长因子和酪氨酸激酶A阳性表达率分别为（41±11）/mm和（45±4）/mm,均明显高于吸烟无COPD组[（28±9）/mm和（20±3）/mm]及对照组[（24±6）/mm和（17±6）/mm],差异均有统计学意义（q值为4.83～7.24,均P＜0.05）;神经生长因子与酪氨酸激酶A的阳性表达率均呈显著正相关（r=0.655,P＜0.05）;神经生长因子和酪氨酸激酶A的阳性表达率与FEV1/FVC呈显著负相关（r值分别为-0.486和-0.665;均P＜0.05）,与吸烟指数无相关性（r值分别为0.282和0.470,均P＞0.05）.结论 神经生长因子和酪氨酸激酶A在COPD患者细支气管上皮细胞中的表达增强,提示其与COPD的疾病进展有关.

Objective To study the expressions of nerve growth factor （NGF） and tyrosine kinase receptor A （TrkA） in bronchiolar epithelial cells of patients with chronic obstructive pulmonary disease （COPD）. Methods Thirty-one patients admitted to Tongji Hospital from January to June in 2008 for incision of lung tissues were included in the study They were divided into 3 groups： （ 1 ） the control goup：9 patients （2 females and 7 males）, average age （52 ± 10） years old, who did not smoke and did not have COPD; （2） the COPD group： 11 patients, all male, average age （61 ± 11 ） years old, who smoked and had a diagnosis of COPD; （3） the smoker group, 11 patients, all male, average age （ 56 ± 6） years old, who smoked but did not have COPD. The pathological changes were detected by HE staining. The localizations and the levels of NGF and TrkA expressions in bronchiolar epithelial cells were observed by immunohistochemical technology on bronchiolar biopsy sections. The analysis of variance （ ANOVA）, the student-Newman-Keuls （SNK） and the Pearson correlation coefficient were used for statistical analysis through SPSS13.0. Results The COPD group exhibited significantly enhanced NGF and TrkA expression levels, and the positive cell numbers were （41 ± 11 ）/mm and （45 ± 4）/mm in bronchiolar epithelial cells respectively, as compared with the other 2 groups [（28 ± 9）/mm, （20 ± 3）/mm and （24 ±6）/mm, （17 ± 6）/mm], q=4. 83 -7.24, all P ＜ 0. 05. A significant positive correlation was found between the expressions of NGF and TrkA in bronchiolar epithelial cells （ r = 0. 655, all P ＜ 0. 05 ＝. A significant negative correlation was found between NGF and TrkA expressions and the levels of FEV1/FVC （ r =- 0. 486, - 0. 665, all P ＜ 0. 05 ＝. Conclusions The expressions of both NGF and TrkA in bronchiolar epithelial cells were significantly increased in COPD patients. It suggested that NGF and TrkA might play an important role in the progress of COPD.