转化生长因子β1及Ki-67核抗原在涎腺黏液表皮样癌中的表达和意义

Expression of transforming growth factor-β1 and Ki-67 nuclear antigen in salivary gland mucoepidermoid carcinoma

目的 研究转化生长因子β1（transforming growth factor-β1,TGF-β1）、Ki-67核抗原在涎腺黏液表皮样癌（mucoepidermoid carcinoma,MEC）中的表达和意义.方法 应用免疫组织化学SP法检测20例涎腺多形性腺瘤（pleomorphic adenoma,PA）、45例涎腺MEC（29例高分化、16例中低分化）组织中TGF-β1和Ki-67核抗原的表达.结果 涎腺MEC中TGF-β1（39/45）、Ki-67核抗原（43/45）的表达高于PA,差异有统计学意义（P＜0.05）;TGF-β1在中低分化MEC中的表达（16/16）高于高分化MEC（23/29）及PA（12/20）,差异有统计学意义（P＜0.05）,而TGF-β1在高分化MEC（6/23）与PA（8/12）中的表达差异无统计学意义（P＞0.05）;在MEC中Ki-67核抗原的表达随TGF-β1表达增强而上升（P＜0.05）,二者呈显著正相关（r=0.55,＜0.001）.结论 TGF-β1的高表达在涎腺MEC的分化及恶性增殖过程中可能起重要作用.

Objective To investigate the expression and significance of transforming growth factor-β1 （TGF-β1）and Ki-67 nuclear antigen in salivary gland mucoepidermoid carcinoma. Methods The expression of TGF-β1 and Ki-67 nuclear antigen in 20 cases of salivary gland pleomorphic adenoma and 45 cases of mucoepidermoid carcinoma were detected by the streptavidin-peroxidase immunohistochemical method. Results Expression of TGF-β1 （39/45） and Ki-67 nuclear antigen （43/45） in mucoepidermoid carcinoma was significantly higher than in pleomorphic adenoma （ P 〈 0.05 ）. TGF-β1 expression was significantly higher in moderately/poorly differentiated mucoepidermoid carcinoma （ 16/16 ） than that in well differentiated mucoepidermoid carcinoma （23/29） and pleomorphic adenoma （12/20） （ P 〈 0.05 ）, but not different between well differentiated mucoepidermoid carcinoma （6/23） and pleomorphic adenoma （8/12）（P 〉 0.05 ）. The expression of Ki-67 was increased with the increment of TGF-β1 expression in mucoepidermoid carcinoma （P 〈 0.05 ）. Conclusions The high expression of TGF-β1 may play an important role in cell differentiation and malignant proliferation of mucoepidermoid carcinoma.