大鼠口腔黏膜急性排斥反应外周血CD68水平与巨噬细胞浸润的相关性研究

Correlation between the CD68 proportion of peripheral blood mononuclear calls and macrophage infffltration during acute rejection of rat oral mucosal xenotransplantation

目的 探讨大鼠外周血CD68单核细胞比例和颊黏膜巨噬细胞浸润变化及二者相关性在口腔黏膜急性排斥反应中的作用和意义.方法 36只雌性Wistar大鼠按随机数字表随机分为移植组（15只）、创伤对照组（12只）及正常对照组（9只）,建立口腔黏膜异种移植模型,分别于术后1、2、4周采用流式细胞术检测各组大鼠外周血CD68单核细胞百分数,免疫组化SP法检测颊黏膜巨噬细胞CD68的表达.结果 移植组3个时点外周血CD68单核细胞百分数呈高低起伏的变化趋势,峰值出现在术后4周（43.1%）,术后2周为最低值（10.4%）;组织学观察显示术后1周巨噬细胞环形包绕密集浸润于移植物周围,术后2周转向浸润于移植物内部,术后4周随移植物坏死崩解巨噬细胞数量减少.术后1周移植组颊黏膜巨噬细胞计数达峰值580.0（195.5）个/高倍视野,随时间推移呈递减趋势[术后2周计数值为220.0（111.9）个/高倍视野,术后4周为173.0（97.7）个/高倍视野].结论 大鼠口腔黏膜急性排斥反应中巨噬细胞可识别并参与排斥异种移植物,移植术后早期外周血单核细胞百分数可反映移植区颊黏膜巨噬细胞浸润情况.

Objective To investigate the role of the dynamic process of peripheral blood CD68 mononuclear cells proportion and macrophages inflitration and possible correlation between them during acute rejection of rat oral mucosal xenotransplantation. Methods Thirty-six female wistar rats were divided into three groups randomly, including xenotransplantation group（ n = 15 ）, trauma control group（ n = 12） and normal control group （ n = 9）. The rat oral mucosa xenotransplantation model was established. The flow cytometry was used to evaluate the peripheral blood CD68 mononuclear cell and immunohistochemical assay performed to detect the macrophages infiltration one week （W1）, two weeks （W2）and four weeks （W4） after xenotransplantation. Results The peripheral blood CD68 mononuclear cells percentage of each xenotransplantation group presented a rise and fall tendency at the three time points, and the peak value appeared at W4（43. 1% ）, and the nadir at W2（ 10.4% ）. The macrophage counts achieved peak value in xenotransplantation group at W1 [ 580.0 （195.5） cell/high power field ], and then reduced with time. Conclusions The mononuclear cells and macrophage were capable of recognizing the xenograft and directly participated the acute rejection of rat oral mucosal xenotransplantation. The peripheral blood mononuclear cells percentage could reflect macrophage infiltrating condition at the early stage of the acute rejection.