应用超高分辨质谱成像技术研究脂类分子在小鼠肝组织中的分布

Imaging and Identification of Phospholipids in Mouse Liver Tissue by Matrix Assisted Laser Desorption Ionization-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Imaging

利用基质辅助激光解吸电离-傅里叶变换离子回旋共振质谱仪对磷脂类分子在小鼠肝组织中的分布进行了研究,建立了质谱成像技术检测小鼠肝组织中磷脂类分子分布的分析方法。以7g/Lα-腈基-4-羟基肉桂酸的50%甲醇溶液(含0.2%三氟乙酸)作为基质,采用正离子采集模式,准确鉴定了5类13种磷脂类分子,其分子量主要分布在700～900Da之间,且观察到它们在组织内分布呈现不均匀性。本研究表明,以超高分辨和超高精度的质谱仪开展组织成像研究,不仅可以探究脂类分子在组织中的分布,而且可以直接准确鉴定相关分子,真正实现分子水平上的组织成像。

Matrix assisted laser desorption ionization-Fourier transform ion cyclotron resonance mass spectrometry（MALDI-FTICR MS）-based mass spectrometric imaging（MSI） was applied to profile and identify phospholipid molecules in mouse liver tissues,which were cut into tissue sections（7 μm） on a cryostat at-20 ℃ and covered with 7 g/L of alpha-cyano-4-hydroxycinnamicacid（CHCA） as matrix in 50% methanol/0.2 trifluoroacetic acid solution.13 phospholipid compounds,which are classified as 5 different species,phosphatidic acid（PA）,phosphatidylcholine（PC）,phosphatidylethanolamine（PE）,phosphatidylglycerol（PG） and phosphatidylserine（PS）,were accurately identified.Their molecular weights are between 700 and 900 Da.The present results indicate that MALDI-FTICR MS-based MSI is a powerful tool to explore the distribution of disease-related molecules and drug metabolites in tissues.