化学发光免疫分析检测人血清中的癌胚抗原

Chemiluminescent Analysis of Carcinoembryonic Antigen in Serum Samples

基于辣根过氧化物酶(HRP)催化H2O2氧化3-(4-羟苯基)丙酸(PHPPA),生成能发荧光的3-(4-羟苯基)丙酸二聚体,在乙腈介质中,在增强剂咪唑参与下,与双[2,4,6-三氯苯基]草酸酯(TCPO)和H2O2反应产生强化学发光。用辣根过氧化物酶(HRP)标记癌胚抗原(CEA)单克隆抗体,通过CEA的双抗夹心免疫反应,建立了简单、灵敏和快速检测人血清中的癌胚抗原(CEA)含量的化学发光免疫分析方法。CEA在1.0～80.0μg/L范围具有良好的线性关系;检出限为0.3μg/L(S/N=3),相对标准偏差(n=11)为3.9%。已成功用于人血清样品中CEA含量的测定。

Based on the catalyzed oxidation of horseradish peroxidase（HRP） to the hydrogen peroxide and 3（4-hydroxyphenyl propionate）（PHPPA） reaction,the fluorescent product,3-（4-hydroxyphenyl propionate） dimer,can react with bis（2,4,6-trichlorophenyl） oxalate（TCPO） and hydrogen peroxide to enhance chemical luminescence by the participation of imidazole enhancer in acetonitrile medium.With horseradish peroxidase（HRP） labeled Carcinoembryonic Antigen（CEA） monoclonal antibody,by ＂sandwich type＂CEA immune response,a simple,sensitive,and rapid chemiluminescence immunoassay method for the determination of Carcinoembryonic Antigen（CEA） in human serum samples has been developed.There was a very good linear correlation between response and amount of CEA in the range of 1.0-80.0 μg/L（r = 0.9989）.The detection limit was 0.3 μ g/L（S/N = 3）.The relative standard deviation（n = 11） was 3.9%.The proposed method has been used for the determination of CEA in human serum.