铜绿假单胞菌的临床分布及耐药性监测

Clinical distribution of pseudomonas aeruginosa and drug resistance

目的探讨铜绿假单胞菌在临床感染中的分布及其耐药性,为临床合理用药提供依据。方法细菌鉴定应用美国MicroScan WalkAway-96SI全自动微生物分析仪,药敏试验采用K-B纸片扩散法,用WHONET5.4软件进行统计分析。结果 2年来临床分离的293株铜绿假单胞菌主要来源于呼吸道标本(73.4%),且主要集中于ICU和神经外科等科室;铜绿假单胞菌对头孢哌酮/舒巴坦的耐药率最低(15.7%)、其次为阿米卡星(18.4%)、亚胺培南(21.5%)、哌拉西林/他唑巴坦(26.6%)、头孢他啶(28.3%)等,而对头孢唑林的耐药率最高(100.0%)。结论铜绿假单胞菌是医院感染的主要病原菌之一,对其加强耐药性监测,指导临床合理应用抗菌药物,控制医院感染的流行十分重要。

OBJECTIVE To investigate the clinical distribution and analyze of the antibiotic resistance of Pseudomhas aeruginosa to provide the basis for clinical medication. METHODS The isolated bacteria were identified by MicroScan WalkAway-96SI and the sensitivity test was performed by adopting Kirby-Bauer method, the data was analyzed by WHONET5. 4 software. RESULTS 73. 4% of P. aeruginosa were isolated from respiratory tract specimen gathered in ICU and neurosurgery department. Among the antibiotics, the resistance rate to cefoperazone/sulbactam was the lowest （15. 7%）, followed by amikacin （18. 4%）, imipenem （21. 5%）, piperacllin/tazobactam （26. 60%） and ceftazidime （28. 30/oo）, but the resistant rate to cefazolin was the highest （100. 0%）. CONCLUSION P. aeruginosa is the major pathogenic bacteria in nosocomial infection. The surveillance to antimicrobial resistance is very important for guiding the rational application of antibiotics and controlling the prevalence of nosocomial infection.