川芎饮片及其煎剂HPLC指纹图谱的相关性研究

Analysis on Correlation of HPLC Fingerprint on Decoction Pieces and Decoction of Rhizoma Ligusticum

目的:研究川芎饮片及其煎剂指纹图谱剂的相关性。方法:采用HPLC分析指纹图谱,色谱柱Zorbax SB-Aq;流动相乙腈-0.05%磷酸溶液(梯度洗脱);检测波长290 nm;流速1 mL·min-1;柱温35℃。结果:川芎饮片标示出20个主要特征峰,10批样品的相似度在0.821～0.994,有两批样品相似度小于0.9。川芎煎剂能检出川芎的16个主要特征峰(峰1～16),10批煎剂的相似度在0.958～0.998,相似度较高。川芎煎剂的16个特征峰面积的提取率在10.67%～111.63%,差异相当大,极性大的成分提取率明显较高,极性小的成分提取率明显较低。饮片与煎剂之间的相似度为0.481～0.970,有两批样品相似度大于0.9。结论:传统煎煮法制备的川芎煎剂基本能检出川芎的大部分特征峰,但特征峰面积的相对比值差异较大,本研究为川芎、川芎煎剂、含川芎复方煎剂的指纹图谱研究提供了一定参考。

Objective：To study the correlation of HPLC fingerprint on the decoction pieces and decoction of Rhizoma Ligusticum.Method：HPLC with Zorbax SB-Aq was used,acetonitrile-0.05% phosphoric acid（gradient elution）as a mobile phase,detection wavelength was at 290 nm,flow rate was 1 mL.min-1,and column temperature was 35 ℃.Result： Twenty common peaks were separated in the decoction pieces of Rhizoma Ligusticum,the similarity of 10 samples was 0.821-0.994.16 common peaks（pesk 1-pesk 16） were separated in the decoction of Rhizoma Ligusticum,the similarity of 10 samples was 0.958-0.998.The extraction percentages of 16 peaks of the decoction were 10.67%-111.63%,there were larger difference.The similarity between the decoction pieces and decoction was 0.481-0.970.Conclusion： The much composition of Rhizoma Ligusticum could be extracted by traditional decoction method.The results can provide a reference for the study of the fingerprint of Rhizoma Ligusticum,Rhizoma Ligusticum decoction or preparations containing Rhizoma Ligusticum.