乳康酊的薄层鉴别及丹皮酚的含量测定

Identification and Determination of Paeonol of Rukang Tincture

目的:建立乳康酊薄层鉴别和含量测定的方法。方法:采用薄层色谱法对乳康酊中徐长卿、龙血竭进行定性鉴别;采用高效液相色谱法测定制剂中丹皮酚的含量。结果:在薄层色谱中可检出徐长卿和龙血竭;丹皮酚在0.03～0.27 g.L-1与峰面积呈良好的线性关系(r=0.999 9),平均加样回收率为99.01%,RSD 2.22%(n=6)。结论:所建立的方法简便、准确、可靠,可用于该制剂的质量控制。

Objective：To establish an identification and conten determination methods of Rukang Tincture.Method：Radix Cynanchi Paniculati and Resina Draconis were identified by TLC.The content of paeonol in Rukang tincture was determined by HPLC.Result：Radix Cynanchi Paniculati and Resina Draconis could be detected by TLC.Determine the content of paeonol by HPLC,paeonol showed a good linear relationship at a range of 0.03-0.27 g·L-1（r = 0.999 9）.The average recovery rate was 99.01% with RSD of 2.22%（n = 6）.Conclusion：The established methods are simple,accurate,reliable and can be used for the quality control of Rukang tincture.