中风皂贝化痰胶囊对大鼠脑缺血再灌注损伤后的神经保护作用及碱性成纤维细胞生长因子表达的影响

Influence of Zhongfeng Zaobei Huatan Capsules on Nerve Protection and Expression of Basic Fibroblast Growth Factor in Rats with Cerebral Ischemia-reperfusion Injury

目的:观察中风皂贝化痰胶囊对大鼠脑缺血再灌注损伤后的神经保护作用及对碱性成纤维细胞生长因子(basicfibroblast growth factor,bFGF)表达的影响。方法:SD大鼠50只随机分成假手术组、缺血再灌注组、中风皂贝化痰胶囊高、中、低剂量组(1.62,1.08,0.54 g·kg-1·d-1),每组10只。采用Zea Longa法制作脑缺血再灌注大鼠模型,观察中风皂贝化痰胶囊对大鼠脑缺血再灌注损伤后的神经功能症状以及bFGF表达的影响。结果:与假手术组相比,缺血再灌注组大鼠出现严重的神经功能缺失症状,皮质和海马区bFGF表达增强;与脑缺血再灌注组相比,中风皂贝化痰胶囊组能显著改善大鼠神经功能缺失症状,且上调皮质和海马区bFGF的表达,其中以中风皂贝化痰胶囊高剂量组的神经保护作用最为显著,bFGF阳性细胞区积分吸光度为153.20±0.76。结论:中风皂贝化痰胶囊对大鼠脑缺血再灌注损伤具有保护作用,其作用机制可能与上调bFGF的表达相关。

Objective：To observe the effect of Zhongfeng Zaobei Huatan Capsules（ZFZBHTC） on nerve protection and the expression of basic fibroblast growth factor（bFGF）in the rats with cerebral ischemia-reperfusion injury.Method：Fifty SD rats were randomly divided into a sham operation group,an ischemia-reperfusion（I /R） group,a high-dose of ZFZBHTC group（1.62 g·kg-1·d-1）,a moderate-dose of ZFZBHTC group（1.08 g·kg-1.d-1）and a low-dose of ZFZBHTC group（0.54 g·kg-1·d-1） with 10 rats in each group.The rat model of cerebral Ischemia-reperfusion was prepared by Zea Longa method.The impacts of ZFZBHTC on neurologic function and the expression of bFGF were observed.Result：Compared with the sham operation group,Group I-R presented severe neurological deficits and the increase of bFGF expression in the cortex and hippocampus.Compared with Group I-R,neurological deficits of rats were relieved significantly and bFGF expression increased distinctly in ZFZBHTC groups,of which the high-dose group presented the most significant effect on nerve protection,and the integrated optical density of positive cells in areas of bFGF was 153.20 ± 0.76.Conclusion：ZFZBHTC acts on the protection of cerebral ischemia-reperfusion injury in rats,which is probably correlated with its increase on bFGF expression.