藏药湿生扁蕾提取物治疗小鼠溃疡性结肠炎的研究

Gentianopsis paludosa Extract for Treatment of Experimental Ulcerative Colitis in Mice

目的:观察藏药湿生扁蕾提取物(GTP)对小鼠实验性溃疡性结肠炎的治疗作用。方法:用三硝基苯磺酸(TNBS)/乙醇灌肠制作小鼠实验性溃疡性结肠炎(UC)模型,小鼠ig给予GTP(1.07,2.14,4.28 g.kg-1),连续14 d,观察对小鼠体质量变化、腹泻、结肠黏膜组织形态学及脾脏指数的影响。结果:湿生扁蕾提取物可不同程度地改善由TNBS的乙醇溶液所致UC小鼠体质量下降及腹泻症状,显著降低UC小鼠大体形态学损伤的肠黏膜粘连及其溃疡分值,明显抑制UC小鼠脾脏肿大。结论:湿生扁蕾提取物对小鼠溃疡性结肠炎有较好的改善作用,能减轻结肠局部的病理性损伤。

Objective： Therapeutic effects of the extract from Gentianopsis paludosa on experimental ulcerative colitis were observed in mice.Method：Experimental ulcerative colitis（UC） was made by trinitrobenzene sulfonic acid（TNBS） /ethanol enema in mice.Animals were fed with GTP（1.07,2.14,4.28 g·kg-1） ig for continuous 14d.Changes in body weight,diarrhea,histomorphology of colonic mucosa and spleen index of morphology were observed.Result： The extract from G.paludosa could improve diarrhea and worsening of body constitution to varying degrees in the animals with UC induced by trinitrobenzenesulfonic acid（TNBS） solution of ethanol,and significantly alleviate gross morphological damage and intestinal adhesion,improve ulcer scores,and significantly inhibit spleen enlargement.Conclusion： The extract from G.paludosa of offers beneficial function for ulcerative colitis in mice,and can reduce pathological damage in colonic tissues.