胃肠舒对胃肠平滑肌细胞一氧化氮释放的影响

Effect of Weichangshu on Release of Nitric Oxide in Gastrointestinal Smooth Muscle Cells

目的:探讨胃肠舒促胃肠动力的作用机制。方法:组织块法原代培养胃肠平滑肌细胞,实验分胃肠舒低、中、高剂量组、西沙必利组和正常对照组,各组分别用受试药物(正常对照组用DMEM干粉培养基)干扰细胞24 h,采用一氧化氮荧光探针(DAF-FMDA)反应30 min,流式细胞仪检测一氧化氮(NO)释放。结果:胃肠舒大、中剂量组与正常对照组比较,胃肠平滑肌细胞NO释放的荧光强度明显减弱(P<0.01,P<0.05),与西沙必利组相似。结论:胃肠舒促胃肠动力的作用机制可能是通过抑制胃肠平滑肌细胞内NO的生成,进而抑制相关酶的活化,拮抗环磷酸鸟苷(cGMP)的作用,从而使胞内Ca2+增多,Ca2+信号系统引发一系列生理功能而促进胃肠平滑肌收缩效应。

Objective：To investigate the mechanisms of Weichangshu in promoting gastrointestinal motility.Method：Gastrointestinal smooth muscle cells were primarily cultured with tissues.Animals were divided into a lowdose of Weichangshu group（LW）,a moderate-dose of Weichangshu group（MW）,a high-dose of Weichangshu group（HW）,a cisapride group（CI） and a normal control group（NC）.The gastrointestinal smooth muscle cells were interfered with tested reagents for 24 h in different groups（normal group with DMEM for Group NC））,and with 3-Amino,4-aminomethy1-2′,7′-difluorescein,diacetate（DAF-FMDA） for 30min for reaction,and release of NO was detected by using a Flow cytometry.Result：Compared with Group NC,the fluorescence intensities of NO release were significantly reduced in Group HW and Group MW（P 0.01,P 0.05）,which were similar to Group CI.Conclusion： Inhibition of generation of NO in gastrointestinal smooth muscle cells followed by inhibition of activation of correlated enzymes to antagonize formation of cGMP,leading to increase of intracellular Ca2 ＋ to trigger series of physiological functions to accelerate gastrointestinal smooth muscle contraction may be involved in the mechanisms of Weichangshu for promoting gastrointestinal motility.