摘要
目的:探讨胃肠舒促胃肠动力的作用机制。方法:组织块法原代培养胃肠平滑肌细胞,实验分胃肠舒低、中、高剂量组、西沙必利组和正常对照组,各组分别用受试药物(正常对照组用DMEM干粉培养基)干扰细胞24 h,采用一氧化氮荧光探针(DAF-FMDA)反应30 min,流式细胞仪检测一氧化氮(NO)释放。结果:胃肠舒大、中剂量组与正常对照组比较,胃肠平滑肌细胞NO释放的荧光强度明显减弱(P<0.01,P<0.05),与西沙必利组相似。结论:胃肠舒促胃肠动力的作用机制可能是通过抑制胃肠平滑肌细胞内NO的生成,进而抑制相关酶的活化,拮抗环磷酸鸟苷(cGMP)的作用,从而使胞内Ca2+增多,Ca2+信号系统引发一系列生理功能而促进胃肠平滑肌收缩效应。
Objective:To investigate the mechanisms of Weichangshu in promoting gastrointestinal motility.Method:Gastrointestinal smooth muscle cells were primarily cultured with tissues.Animals were divided into a lowdose of Weichangshu group(LW),a moderate-dose of Weichangshu group(MW),a high-dose of Weichangshu group(HW),a cisapride group(CI) and a normal control group(NC).The gastrointestinal smooth muscle cells were interfered with tested reagents for 24 h in different groups(normal group with DMEM for Group NC)),and with 3-Amino,4-aminomethy1-2′,7′-difluorescein,diacetate(DAF-FMDA) for 30min for reaction,and release of NO was detected by using a Flow cytometry.Result:Compared with Group NC,the fluorescence intensities of NO release were significantly reduced in Group HW and Group MW(P 0.01,P 0.05),which were similar to Group CI.Conclusion: Inhibition of generation of NO in gastrointestinal smooth muscle cells followed by inhibition of activation of correlated enzymes to antagonize formation of cGMP,leading to increase of intracellular Ca2 + to trigger series of physiological functions to accelerate gastrointestinal smooth muscle contraction may be involved in the mechanisms of Weichangshu for promoting gastrointestinal motility.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第2期121-124,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30873263)
山东省自然科学基金项目(ZR2009CL001)
关键词
胃肠舒片
胃肠平滑肌细胞
一氧化氮荧光探针
流式细胞仪
Weichangshu tablets
gastrointestinal smooth muscle cells
3-Amino
4-aminomethy1-2′
7′-difluorescein
diacetate(DAF-FMDA)
flow cytometry