补阳还五汤对老龄大鼠红细胞膜组分的影响

Effect of Buyang Huanwu Decoction on Components of Erythrocyte Membranes in Aged Rats

目的:从红细胞膜组分的变化探讨补阳还五汤改善老龄大鼠血液黏度的作用机制。方法:40只Wistar大鼠,随机分为轻龄对照组、模型组、补阳还五汤高、低剂量组,每组10只。给药19 d后,取血测定血液流变学指标以及红细胞膜组分相关指标。结果:老龄大鼠血液黏度明显高于轻龄大鼠,而补阳还五汤可明显降低老龄大鼠的全血黏度和血浆黏度,同时可明显降低红细胞膜上胆固醇和丙二醛(MDA)含量,提高红细胞变形能力和红细胞膜上唾液酸含量、超氧化物歧化酶(SOD)活性。结论:补阳还五汤降低血液黏度的机制可能与影响红细胞膜上脂质代谢,提高抗氧化能力,减少氧自由基生成,以及改善红细胞膜上的负电荷荷电量等方面有关。

Objective： To investigate the mechanisms of Buyang Huanwu Decoction（BYHWD） in improving blood viscosity in aged rats by observing changes of the components of erythrocyte membranes.Method： Forty Wistar rats were randomly divided to a young control group,a model group,high-dose of BWD group and a low-dose of BYHWD group with 10 rats in each group.The related indexes in hemorheology and the components of erythrocyte membranes were determined 19 days after giving medicine.Result： Blood viscosity of aged rats was higher than young rats obviously.The whole blood viscosity and plasma viscosity of aged rats were decreased by BWD obviously.The contents of cholesterol and MDA in erythrocyte membranes were decreased.Red blood cell deformability,and the contents of sialic acid and SOD in erythrocyte membranes were increased.Conclusion： Decrease of blood viscosity by BYHWD may be related to its effect on metabolism of lipid,enhancement of ability of anti-oxidation,decrease of the contents of oxygen radicals and improvement of the carrying capacity of negative charge in erythrocyte membranes.