小儿肺热咳喘口服液对甲型H1N1流感病毒感染小鼠的影响

Effect of Xiaoer Feire Kechuan Oral Solution on Mice Infected by Influenza Virus A (H1N1)

目的:观察小儿肺热咳喘口服液对甲型H1N1流感病毒感染小鼠的影响。方法:以甲型H1N1流感病毒滴鼻感染ICR小鼠造成肺炎模型,造模同时开始ig小儿肺热咳喘口服液20,10,5 mL.kg-1,以达菲为阳性对照,观察肺部炎症、计算肺指数,死亡率和生命延长率,酶联免疫法检测血清中细胞因子的含量。结果:小儿肺热咳喘口服液治疗性给药后能明显降低流感病毒FM1株和PR8株感染后小鼠的肺指数、降低死亡率、延长平均存活天数,而对小鼠血清中的细胞因子含量没有明显影响。结论:小儿肺热咳喘口服液对甲型H1N1流感病毒感染小鼠造成的病毒性肺炎具有明显的治疗作用。

Objective：To observe the effect of Xiaoer Feire Kechuan oral solution on the mice infected by influenza virus A（H1N1）.Method：ICR mice were infected by influenza A（H1N1） with nasal drops（20,10,5 mL.kg-1）,the lung index,death rate,death protection rate and life prolongation were observed and the levels of cytokines in blood serum were detected by ELISA.Result：With treatment of Xiaoer Feire Kechuan oral solution,the lung index,death rate of ICR mice were decreased significantly,while the life span of mice was prolonged.There was no remarkable effect of Xiaoer Feire Kechuan oral solution on the cytokine levels in serum.Conclusion：Xiaoer Feire Kechuan oral solution could inhibit viral pneumonia for mice infected by influenza virus A（H1N1）significantly.