夏龙方对人胃癌SGC-7901细胞黏附和侵袭的影响

Eeffects of Xialongfang on Adhesion and Migration in SGC-7901 Gastric Carcinoma Cells

目的:观察夏龙方对胃癌细胞SGC-7901黏附、侵袭、移动等转移生物学行为的影响。方法:体外培养SGC-7901细胞,分为夏龙方50,100,200 mg·L-1(低、中、高剂量)组和对照组,采用Cytoselect 48-Well Cell Adhesion Assay检测胃癌细胞与基质黏附能力;利用Transwell侵袭转移模型评价夏龙方对胃癌细胞侵袭能力的影响、划痕实验观察夏龙方对SGC-7901细胞移动能力的影响。结果:终浓度100,200 mg·L-1夏龙方能显著降低SGC-7901细胞黏附能力,50～200 mg·L-1可抑制SGC-7901细胞侵袭,终浓度50 mg·L-1夏龙方可显著抑制SGC-7901细胞迁移。结论:夏龙方可降低SGC-7901细胞的黏附能力,侵袭能力,抑制细胞迁移。

Objective：To investigate the influence of Xialongfang on adhesion,invasion and migration in SGC-7901 gastric carcinoma cells.Method：Human gastric carcinoma SGC-7901 cells were cultured in vitro,and treated with different concentration Xialongfang（0-200 mg·L-1）.Cell adhesion was detected with cytoselect 48-well cell adhesion assay.Cell invasion was analyzed with Transwell invasion assay.Cell migration was detected with scratch wound healing assay.Result：50-200 mg·L-1 Xialongfang significantly inhibited the invasive capacities of SGC-7901 cells and inhibited cell adhesion at 100-200 mg·L-1.Wound healing assays showed a significant reduction in the migrated cells after treated for 24 h or 48 h at 50 mg·L-1 Xialongfang.Conclusion：Xialongfang significantly decreased the adhesion and invasive capacities of SGC-7901 cell lines and inhibited cell migration.