齿叶白鹃梅叶对小鼠糖耐量的影响及降糖作用研究

Effects of Leaves of Exochorda serratifolia on Glucose Tolerance in Mice and Hypoglycemic Effect on STZ Induced Diabetic Mice

目的:探讨齿叶白鹃梅叶对正常和糖尿病小鼠糖耐量的影响以及对STZ诱导糖尿病小鼠的降糖作用。方法:采用腹腔注射链脲佐菌素(150 mg·kg-1)诱导小鼠造成糖尿病模型,先观察齿叶白鹃梅叶水提物和80%醇提物对正常和糖尿病小鼠糖耐量的改变;再观察齿叶白鹃梅叶的水提取物和80%醇提物物以及阳性药盐酸二甲双胍(333 mg·kg-1)对小鼠血糖值的影响。结果:糖耐量实验表明齿叶白鹃梅叶水提物和80%醇提物能降低正常和糖尿病小鼠餐后1,2 h血糖,并且齿叶白鹃梅水提物对糖尿病小鼠餐后2 h血糖具有显著降低作用(P<0.05);降血糖药理实验表明齿叶白鹃梅叶的水提物高剂量与醇提取物高剂量均能降低STZ致糖尿病小鼠的血糖,且齿叶白鹃梅叶水提物高剂量组的降糖作用优于齿叶白鹃梅叶醇提物高剂量组的降糖作用。结论:齿叶白鹃梅叶能改善正常和链脲佐菌素糖尿病小鼠糖耐量,并具有一定的降糖作用。

Objective：To investigate the effects of leaves of Exochorda serratifolia on the glucose tolerance in normal and diabetic mice and to study the hypoglycemic effect on STZ induced diabetic mice.Method：First,diabetic mice model was induced by STZ（150 mg·kg-1）.Normal or diabetic mice were given a single dose of the water extract from leaves and the 80% EtOH extract from leaves,and the changes of glucose tolerance were observed respectively;Second,The water extract and the 80% EtOH extract was administrated at different does（9.5,3.8 g·kg-1） to the STZ induced mice for 10 days.The mice of positive control group were given meformin hydrochloride（333 mg·kg-1）.And then,the levels of blood glucose were inspected.Result：The water extract and the 80% EtOH extract could lower the serum glucose levels 1 h and 2 h after glucose administration in normal or diabetic mice.And the water extract could lower the the serum glucose levels 2 h after given glucose in diabetic mice（P0.05）;The blood glucose level was lower remarkably in the hyperglycemia mice that were administrated at high dose of the water extract of leaves of E.serratifolia（P0.05）.Conclusion：The leaves of E.serratifolia could improve the glucose tolerance in normal and diabetic mice induced by STZ and also had an hypoglycemic effect on STZ induced diabetic mice.