远场超分辨随机光重建显微镜(STORM)研究进展

Progress in far-field super-resolution stochastic optical reconstruction microscopy(STORM)

了解细胞内分子尺度的动态和结构的特征是生命科学迫切需要解决的问题,要求远场光学成像要求纳米或亚纳米量级的空间分辨率。介绍了一种实现打破衍射极限的远场荧光显微成像技术——随机光重建显微术(STORM),其分辨率可以达到横向分辨率20 nm,轴向分辨率50 nm,理论上这种方法的空间分辨率可以达到单分子定位的精度。具体介绍了其成像的基本原理,在三维、多色成像方面的进展,和目前面临的问题及今后的发展方向。

Understanding intracellular molecule-scale characteristic of dynamics and structures is urgently demanded to solve issues in today＇s life science.In order to solve this problem,a far field optical imaging obtained nanometer or sub-nanometer scale 3D resolution will be demanded.The far-field fluorescence microscopy,which broken diffraction barrier,Stochastic Optical Restructure Microscopy（STORM） is introduced.The STORM can be achieved resolution of 20 nm laterally and 50 nm axially.In theory,the STORM can be achieved single molecule location precision.Imaging foundational principle,progress of 3D and multi-color imaging,recently faced challenge as well as the direction of development about the STORM is talked in detailed.