万通酱油HPLC指纹图谱的研究及其在真伪鉴别中的应用

The study on HPLC finger-print of Wantong soy sauce and the application in identification

试验摸索出建立万通酱油HPLC指纹图谱共有模式的最佳试验方案,即色谱柱:ODS-C18(250 mm×4.6 mm,5μm);流动相:CH3OH-0.02 mol/L K2HPO4溶液(2∶98);柱温:室温;检测波长210 nm;流速为0.8 mL/min。在该条件下,样品经C18柱脱色处理后,30 min内就完成了HPLC指纹图谱的检测。应用指纹图谱相似度评价系统软件对各样品图谱信息进行处理,选出10批优质正品万通酱油样品生成酱油HPLC指纹图谱共有模式,从中选出7个重复性好的色谱峰作为万通酱油HPLC指纹图谱的特征峰。以相似度大小作为判定万通酱油质量合格与否的标准,将生成的对照指纹图谱与伪品酱油HPLC指纹图谱进行比较,即可判断出真伪。

Studied the samples of Wantong soy sauce by HPLC to establish the mutual mode of HPLC finger-print,Chromatographic column： ODS-C18（250 mm×4.6 mm,5 μm）;mobile phase： CH3OH-0.02 mol/L K2HPO4 solution（2∶98）;Temperature as same as room temperature;Detection wavelength： 210 nm;flew velocity： 0.8 mL/min.On the condition of selected chromatographic,it was completed in 30 minutes.By the method and a software ＂Similarity Evaluation System for Chromatographic Fingerprint of TCM＂,we selected 10 batches of certified Wantong soy sauce samples and developed the mutual mode of HPLC finger-print,from which to choose 7 good repeatability chromatographic peaks as the characteristic peak.With similarity degree as the standard of quality identification for Wantong soy sauce,and compare with HPLC finger-print of adulterants,we can identify the authenticity and pseudo graph.