响应面法优化微波辅助提取红甜菜色素的工艺

Response surface methodology for optimizing microwave-assisted extraction of betalain from Beta vulgaris esculenta

在单因素实验基础上,选取液料比、微波功率和微波时间三个因素,采用响应面法,以甜菜红色素溶液吸光值为响应值,对其工艺进行了优化。结果表明,微波时间对吸光值影响最大,其次是微波功率,液料比影响最小。微波辅助提取红甜菜中甜菜红色素的最佳工艺参数为:液料比47.2∶1、微波功率367.8 W、微波时间94.2 s,在此条件下甜菜红色素吸光值的预测值可达0.716,而验证优化工艺参数得到吸光值为0.735,与模型预测值非常接近,采用响应面法对微波辅助提取红甜菜中甜菜红色素的提取条件进行优化合理可行。

On the basis of single-factor test,using response surface methodology（RSM） for optimizing extraction of betalain investigate the effects of solvent/material,microwave output power and microwave time.The results showed that Among the three factors,the microwave time most significantly affects on the absorbance,followed by the microwave output power and the solvent/material.The optimal parameters are as follows： solvent/material 47.2∶1,microwave output power 367.8 W and microwave time 94.2 s,the absorbance is 0.716.Under such conditions,the actual value is 0.735 which is consistent with the prediction walue.This reveal the applicability of RSM to the optimization of extraction of betalain from Beta vulgaris esculenta.