富锗大麦苗安全性评价

Evaluation of the Safety of Germanium-enriched Barley Seedlings

目的:通过研究富锗大麦苗对小鼠的毒理作用,对其进行安全性评价。方法:用不同剂量富锗大麦苗汁连续灌胃小鼠20d,通过急性毒性实验、骨髓细胞微核实验和骨髓细胞染色体畸变实验,测定其对小鼠急性毒理和遗传毒理作用。结果:各实验组富锗大麦苗对小鼠经口半数致死量(LD50)大于1g/mL,动物体质量增长正常,未表现中毒现象;骨髓微核发生率和染色体畸变率均在正常范围内与生理盐水对照组比较无显著差异(P>0.05)。结论:本实验中无受试小鼠中毒死亡现象,没有出现致畸突变现象,提示富锗大麦苗在本实验使用剂量范围内对小鼠不具有急性毒性和遗传毒性,是安全可靠的。

Objective：Safety evaluation of germanium-enriched barley seedlings（EGBS） was conducted by investigating the toxicological effect of EGBS in mice.Methods：The acute toxic effect of EGBS in healthy mice orally administered different doses of EGBS for 20 successive days was evaluated,and for the evaluation of genetic toxic effect,mouse bone marrow micronucleus and chromosomal aberration tests were used.Results：All EGBS dose groups presented a median lethal dose（LD50）1 g/mL and normal animal body weight gain,without any symptoms of poisoning.The incidences of mouse bone marrow micronucleus and chromosomal aberration in all EGBS dose groups were varied in a normal range and did not exhibit a significant difference（P0.05） as compared with the control group（treated with normal saline）.Conclusion：No phenomena of mouse death and chromosomal aberration observed in this experiment suggest that EGBS have no acute and genetic toxic effects on mice and is safe in the tested dose range.