三棱黄酮抗HeLa宫颈癌:降低分裂期细胞比率诱导细胞凋亡

Suppressive Effect of Rhizoma sparganii Flavonoids on HeLa Cells (I):Induce M Stage Arrest and Cell Apoptosis

目的:研究三棱的总黄酮(Rhizoma sparganii flavonoids,RSF)对HeLa宫颈癌细胞的毒理作用及其作用机制。方法:分别检测用含有质量浓度11.7～750μg/mL RSF的DMEM培养基培养时,组间HeLa细胞的增殖活性差异,通过免疫荧光技术、流式细胞术和核型分析方法分析RSF诱导HeLa细胞毒理的机制。结果:RSF含量高于188μg/mL的DMEM培养基可呈剂量性抑制HeLa细胞的增殖活性(P<0.01)、增加细胞凋亡小体的比率(P<0.01);与1%甲醇对照组相比,RSF-375(RSF含量为375μg/mL)与RSF-750(RSF含量为750μg/mL)药物组HeLa细胞的分裂(M)期细胞比率显著下降(P<0.01),细胞间的接触生长状态消失,体积增大;在本次实验中,RSF-375与RSF-750药物组HeLa细胞微管(a-tublin)细胞骨架形态的特异性免疫结果与对照组相比,细胞形态呈显著不规则状改变,微丝突触增多,表现为较高的迁移运动趋势。结论:RSF是三棱抗HeLa宫颈癌的有效成分,在本实验中,RSF可以通过干扰细胞有丝分裂相关的机制显著抑制HeLa细胞的增殖活性(P<0.01)并诱导细胞凋亡(P<0.01)。

Objective：To explore the toxicity effect and mechanisms of Rhizoma sparganii flavonoids（RSF） on HeLa cells.Methods：The effect of RSF at a concentration range of 11.7 to 750μg/mL on the proliferation of HeLa cells was evaluated.The toxic mechanisms were elucidated by immunofluorescence,flow cytometry and karyotype analysis.Results：RSF at a concentration higher than 188μg/mL could significantly（P0.01） inhibit the proliferation of HeLa cells in a dose-dependent manner,and increase the proportion of apoptotic bodies（P0.01）.Compared with the control group,the RSF treatment groups at doses of 375μg/mL（RSF-375） and 750μg/mL（RSF-750） revealed a significant decrease of HeLa cells number at M stage（P0.01）,disappearance of intracellular interaction and an increase of cell volume.In addition,RSF-375 and RSF-750 also showed irregular morphological changes,an increase of synaptics and higher moving trend of microfilament,which were observed in monitoring the specific immnuofluorescence of microtubule protein.Conclusion：RSF is a group of bioactive components against HeLa cervical cancer cells,with the physiological functions of significantly inhibiting proliferation and inducing apoptosis by interfering the mitosis of HeLa cells.