体外消化/Caco-2细胞模型评价面粉锌生物利用率

Assessment of Zn Bioavailability in Wheat Flours Using an in vitro Digestion/Caco-2 Cell Model

采用体外消化/Caco-2细胞模型对10种小麦粉进行锌生物利用率比较。以锌吸收率(Caco-2细胞中的锌含量占加入到细胞的面粉消化液总锌量比例)反映锌生物利用率(Zn bioavailability,Zn BV)。发现不同面粉间锌生物利用率差异具有统计学意义,出粉率78%的安阳中优9507是面粉锌吸收率和可吸收锌量都高的品种,它将进入下一步人体实验进行营养功效的验证。

Zinc bioavailability in 10 wheat flour varieties was assessed by using an in vitro/Caco-2 cell culture model to provide scientific foundation for further human trials and reach nutritional purpose in wheat breeding.Zn bioavailability（Zn BV） was defined as the percentage of the amount of zinc applied to Caco-2 cell monolayer and was consumed by the cells.Significant differences were detected in Zn BV among 10 wheat flour varieties,among which Zhongyou 9507 from Anyang had the highest Zn BV,which would be further tested in human trials for nutritional function study.