黄芩素分子印迹整体柱的制备研究

Preparation of baicalein molecularly imprinted monolithic columns

目的:制备黄芩素分子印迹整体柱并研究其特异识别能力。方法:以黄芩素分子为模板,甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,DMF和十二醇为混合致孔剂,偶氮二异丁腈(AIBN)为引发剂,采用原位聚合法合成黄芩素分子印迹整体柱,并考察了印迹整体柱在不同有机相中对黄芩素与汉黄芩素的识别能力,以及流速、柱温对保留因子与分离因子的影响。结果:模板分子与功能单体之间的比例以1∶6为最佳,得到的分子印迹整体柱对模板分子具有特异的识别能力,黄芩素和其结构类似物汉黄芩素达到较好的分离。结论:合成的印迹整体柱对模板分子具有很强的亲和力和良好的识别能力,可以用做黄芩素的分离材料。

Objective：To prepare a baicalein molecularly imprinted monolithic column and study its specific recognition ability.Methods：A baicalein molecularly imprinted monolithic column was prepared by in-situ polymerization using methacrylic acid（MAA） as a functional monomer,ethylene glycol dimethacrylate（EDMA） as a crosslinking agent,the mixture of dimethylformamide（DMF） and dodecanol as porogenic reagent,and 2,2′-azobisisobutyronitrile（AIBN） as an initiator.The specific recognition ability for baicalein and wogonin was studied in different mobile phase.Effects of flow rate and temperature on retention and separation factor were investigated.Results：The best ratio of template and functional monomer was 1∶6.The baicalein molecularly imprinted monolithic polymer has specific recognition ability for the baicalein molecule.Baicalein and wogonin were separated successfully.Conclusion：The prepared molecularly imprinted monolithic column can be used as the separation media of baicalein with high affinity and good specific recognition ability.