HPLC-DAD-ELSD法同时测定酸枣仁中斯皮诺素、酸枣仁皂苷A和B的含量

HPLC-DAD-ELSD simultaneous determination of spinosin,jujuboside A and jujuboside B in Semen Ziziphi Spinosae(Suanzaoren)

目的：建立同时测定酸枣仁中斯皮诺素、酸枣仁皂苷A和B含量的HPLC-DAD-ELSD方法。方法：采用Grace BravaC18-BDS（250 mm×4.6 mm,5μm）色谱柱,以乙腈（A）-水（B）为流动相进行梯度洗脱,流速1.0 mL.min-1,二极管阵列检测器的检测波长为335 nm,蒸发光散射检测器的漂移管温度为100℃,载气流速为2.9 L.min-1,柱温为25℃,。结果：斯皮诺素、酸枣仁皂苷A和B的线性范围分别为59.40~594.0μg.mL-1（r=0.9996）,49.50~900.0μg.mL-1（r=0.9998）,20.36~407.2μg.mL-1（r=0.9999）;平均加样回收率（n=6）分别为96.9%（RSD=1.8%）,101.2%（RSD=2.5%）,100.3%（RSD=2.9%）。结论：该方法准确、可靠,在同一色谱条件下实现多指标成分的同时测定,为酸枣仁的全面质量评价提供参考。

Objective：To establish an HPLC-DAD-ELSD method for the simultaneous determination of spinosin,jujuboside A and jujuboside B in Semen Ziziphi Spinosae.Methods：The assay was performed on a Grace Brava C18-BDS（250 mm×4.6 mm,5 μm） column by a gradient elution program with acetonitrile-water as mobile phase at a flow rate of 1.0 mL·min-1.The detection wavelength of DAD was 335 nm and the evaporator tube temperature of ELSD was set at 100 ℃ with the nebulizing gas flow-rate of 2.9 L·min-1.The temperature of column was kept at 25 ℃.Results：The linear ranges of spinosin,jujuboside A and jujuboside B were 59.40-594.0 μg·mL-1（r=0.9996）,49.50-900.0 μg·mL-1（r=0.9998）,20.40-407.2 μg·mL-1（r=0.9999）,respectively.The average recoveries（n=6） were 96.9%（RSD=1.8%）,101.2%（RSD=2.5%）,100.3%（RSD=2.9%）,respectively.Conclusion：The method is accurate and reliable for simultaneous determination of multiple index components under the same chromatographic condition,and provides a reference for comprehensive quality evaluation of Semen Ziziphi Spinosae.