摘要
目的:建立同时测定酸枣仁中斯皮诺素、酸枣仁皂苷A和B含量的HPLC-DAD-ELSD方法。方法:采用Grace BravaC18-BDS(250 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-水(B)为流动相进行梯度洗脱,流速1.0 mL.min-1,二极管阵列检测器的检测波长为335 nm,蒸发光散射检测器的漂移管温度为100℃,载气流速为2.9 L.min-1,柱温为25℃,。结果:斯皮诺素、酸枣仁皂苷A和B的线性范围分别为59.40~594.0μg.mL-1(r=0.9996),49.50~900.0μg.mL-1(r=0.9998),20.36~407.2μg.mL-1(r=0.9999);平均加样回收率(n=6)分别为96.9%(RSD=1.8%),101.2%(RSD=2.5%),100.3%(RSD=2.9%)。结论:该方法准确、可靠,在同一色谱条件下实现多指标成分的同时测定,为酸枣仁的全面质量评价提供参考。
Objective:To establish an HPLC-DAD-ELSD method for the simultaneous determination of spinosin,jujuboside A and jujuboside B in Semen Ziziphi Spinosae.Methods:The assay was performed on a Grace Brava C18-BDS(250 mm×4.6 mm,5 μm) column by a gradient elution program with acetonitrile-water as mobile phase at a flow rate of 1.0 mL·min-1.The detection wavelength of DAD was 335 nm and the evaporator tube temperature of ELSD was set at 100 ℃ with the nebulizing gas flow-rate of 2.9 L·min-1.The temperature of column was kept at 25 ℃.Results:The linear ranges of spinosin,jujuboside A and jujuboside B were 59.40-594.0 μg·mL-1(r=0.9996),49.50-900.0 μg·mL-1(r=0.9998),20.40-407.2 μg·mL-1(r=0.9999),respectively.The average recoveries(n=6) were 96.9%(RSD=1.8%),101.2%(RSD=2.5%),100.3%(RSD=2.9%),respectively.Conclusion:The method is accurate and reliable for simultaneous determination of multiple index components under the same chromatographic condition,and provides a reference for comprehensive quality evaluation of Semen Ziziphi Spinosae.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第1期30-33,共4页
Chinese Journal of Pharmaceutical Analysis
基金
山西省卫生厅科技攻关项目(200939)