UFLC法测定母丁香中丁香酚和2-羟基-4,6-二甲氧基-5-甲基苯乙酮的含量

UFLC determination of eugenol and 2-hydroxy-4,6-dimethoxy-5-methylacetophenone in Eugenia caryophyllata Thunb.

目的：建立母丁香中丁香酚和2-羟基-4,6-二甲氧基-5-甲基苯乙酮的超快速液相色谱（UFLC）含量测定方法,完善母丁香药材的质量标准。方法：采用Shim-pack XR-ODSⅡ（3.0 mm×75 mm,2.2μm）,柱温50℃,流动相为甲醇-0.5%磷酸水溶液（60∶40）,流速0.8 mL.min-1,检测波长280 nm。结果：丁香酚和2-羟基-4,6-二甲氧基-5-甲基苯乙酮进样量分别在9.98~79.84μg和17.08~136.64μg范围内与峰面积呈良好的线性关系（r=0.9999）,平均加样回收率（n=5）分别为99.57%和98.68%。结论：母丁香药材的UFLC含量测定法快速、准确、简便、可靠,是一种值得推广的中药质量评价方法。

Objective：To a ultra-fast liquid chromatography（UFLC） method for the determination of eugenol and 2-hydroxy-4,6-dimethoxy-5-methylacetophenone in Eugenia caryophyllata.Methods：Shim-pack XR-ODS Ⅱ（3.0 mm×75 mm,2.2 μm） column was adopted with methanol-0.5% phosphoric acid water solution（60∶40） as the mobile phase at a flow rate of 0.8 mL·min-1;The detection wavelength was 280 nm and column temperature was 50 ℃.Results：Both eugenol and 2-hydroxy-4,6-dimethoxy-5-methylacetophenone showed a good linear relationship with peak area within the range of 9.98-79.84 μg（r=0.9999） and 17.08-136.64 μg（r=0.9999）,respectively;The average recoveries（n=5） were 99.57% and 98.68%.Conclusion：The UFLC method that is used to quantify eugenol and 2-hydroxy-4,6-dimethoxy-5-methylacetophenone is simple,rapid,accurate,and good reproducibility,so it can be used to evaluate the quality of Eugenia caryophyllata;UFLC method can save analysis time and solvent consumption,thus it can be worthy of promotion to quality the traditional Chinese medicine.