不同生长期独一味中山栀苷甲酯和8-O-乙酰山栀苷甲酯的含量研究

Content research of shanzhiside methyl ester and 8-O-acetylshanzhiside methyl ester in Lamiophlomis rotata(Benth.) Kudo with different growing periods

目的：研究分析不同生长期独一味中山栀苷甲酯和8-O-乙酰山栀苷甲酯含量。方法：采用HPLC反相色谱柱Waters Symmetry C18（5μm,4.6 mm×250 mm）,梯度洗脱[0~10 min,乙腈-水（10∶90）;10~30 min,乙腈-水（18∶82）],流速1.0mL.min-1,检测波长235 nm,柱温25℃。结果：不同生长期的独一味中山栀苷甲酯和8-O-乙酰山栀苷甲酯的总含量有明显差异,从6月份到9月份呈近似于正态分布的趋势,以7月中旬到8月初期间的含量为最高。结论：本方法结果准确,重现性好,易于操作,可用于不同生长期独一味中山栀苷甲酯和8-O-乙酰山栀苷甲酯的含量研究。

Objective：To research the contents of shanzhiside methyl ester and 8-O-acetylshanzhiside methyl ester in Lamiophlomis rotata（Benth.）Kudo with different growing periods.Methods：HPLC analysis was performed on Symmetry C18 column,the mobile phase consisted of acetonitrile-water with gradient elution[0-10 min,acetonitrile-water（10∶90）,10-30 min,acetonitrile-water（18∶82）] at the flow rate of was 1.0 mL·min-1,the detection wavelength was 235 nm and the column temperature was 25 ℃.Results：The total content of shanzhiside methyl ester and 8-O-acetylshanzhiside methyl ester in Lamiophlomis rotata（Benth.）Kudo was gaussian distribution from June to September,and the maximum value was in between mid-july and early August.Conclusion：This method is accurate,precise,sensitive and specific to be used in the content determination of shanzhiside methyl ester and 8-O-acetylshanzhiside methyl ester content in Lamiophlomis rotata（Benth.）Kudo with different growing periods.