用SSR标记分析福建漳浦野生稻的遗传多样性

Genetic Diversity of Oryza rufipogon Griff. in Zhangpu County of FuJian Province by Using SSR Markers

应用平均分布于水稻基因组的21对SSR引物,对福建漳浦野生稻、海南野生稻共计62份材料的遗传多样性进行分析。结果表明:漳浦野生稻具有较高的遗传多样性,21个位点共检测到74个等位变异,平均等位变异数A=3.5238,有效等位变异数Ae=2.0629,平均期望杂合度He=0.4635,实际观察杂合度Ho=0.2465,香农指数I=0.8286;根据固定指数(F=0.3887)估算的异交率t=0.4308,说明漳浦野生稻的繁育系统属于一种自交率较高的混合交配类型;分化程度石潭湖群体高于古塘群体。

21 SSR primers were used to assess the genetic diversity of 62 common wild rice individuals from Zhangpu County of Fujian Province and Hainan Province and The results indicated that common wild rice in Zhangpu County Fujian Province had a high level of genetic diversity.74 alleles were detected from 21 loci,A=3.5238,Ae=2.0629,He=0.4635,Ho=0.2465,I=0.7918.t estimated by the fixation index（F=0.4304）was 0.3982,it suggested that the mating system of common wild rice in Zhangpu County of Fujian Province was a type of cross-pollinate system with a high self-breeding rate.For degree of differentiation,Shitanhu population was higher than Gutang population.