陇东野生紫花苜蓿的核型分析

Karyotype Analysis in Wild Medicago sativa L.

采用0.15%秋水仙素和0.002mol/L8-羟基喹啉的混合液对陇东野生紫花苜蓿萌发种子的根尖预处理3h,室温条件下用1%果胶酶和1%纤维素酶酶解1.5h,得到清晰染色体制片。核型分析结果显示,陇东野生紫花苜蓿染色体数目为32条,具有1对随体,为2B核型,染色体核型组成公式为2n=32=30m(2SAT)+2sm,染色体长度组成公式为2n=2L+18M2+8M1+4S。

The chromosome flaking of root-tip on germinate seed in wild Medicago sativa L.can be made clearly,which were pretreated by the mixture of 0.15% colchicine and 0.002mol/L 8-hydroxyquinoline for 3h,and then,enzymolysissed by 1% cellulase＋1% pectinase for 1.5h at 25℃.The result of karyotype analysis shows that the total of chromosome in wild Medicago sativa L.is 32 with a pair of satellites.The karyotype of such specie is 2B type and the karyotype formulation is 2n=32=30m（2SAT）＋2sm,the length construction is 2n=2L＋18M2＋8M1＋4S.