Fas、FasL在非小细胞肺癌中的表达及其与肿瘤免疫和细胞凋亡的关系

Expression of Fas/FasL protein and its relationship with tumorigenesis immunology and apoptosis in non-small cell lung cancer

目的为探讨Fas/FasL系统在非小细胞肺癌(NSCLC)发生发展中所起的免疫逃逸作用,为临床早期诊断肺癌、判断预后、设计新的治疗策略提供依据。方法采用免疫组织化学方法检测Fas、FasL在NSCLC组织及正常肺组织中的表达,同时采用抗CD45RO抗体检测肿瘤组织中浸润淋巴细胞(TIL)的数量,应用TUNEL技术检测肺癌细胞凋亡。结果 Fas在肺癌组织低表达并存在异位表达,表达率与细胞分化有关;FasL在肺癌组织中高表达,表达率和细胞类型及淋巴结转移相关,与TIL浸润程度呈负相关,Fas表达与肺癌细胞凋亡水平无关。结论 NSCLC肺癌组织中普遍存在Fas表达的下调、异位表达及FasL高表达,导致癌细胞的免疫逃逸,参与NSCLC的发生和发展。

Objective In order to provide evidence for early diagnosis,prognosis prediction and new treatment of lung cancer,we analyze the immune escape of the Fas/FasL system in the carcinogenesis and progress of non-small cell lung cancer（NSCLC）.Methods Immunohisochemistry（IHC） method was used to determine the expression of Fas/FasL protein in NSCLC and normal lung tissues;CD45RO antibody and TUNEL technique were used to detect the tumor infiltrating lymphocytes（TILs） and apoptosis respectively.Results Fas expression was found to be significantly reduced in cancer tissues,and it was correlated with the degree of tissue differentiation.There was a high level of FasL expression in cancer tissues.The expression rate of FasL was correlated with the cell type and lymph node metastasis,and negatively correlated with TIL infiltration degree.Fas expression had no association with the apoptosis level of cancer cells.Conclusion Reduced or absent expression of Fas and increased FasL expression are common in NSCLC tissues,which leads to the escape of cancer cells from immunosurveillance and thus the carcinogenesis and development of NSCLC.