春小麦抗蚜性与麦长管蚜EST活性及GSH活性的关系

Effect of Aphid Resistance of Wheat on Esterase and Glutathiones S-transferases Activity of Female Apterae(Sitobion avenae F.)

为阐明春小麦对蚜虫的抗生性机理及蚜虫对寄主的适应性机制,选用抗蚜性不同的8个春小麦品种(系)为试验材料,利用灌浆期人工接种的方法,研究了小麦抗蚜水平与麦长管蚜无翅型孤雌胎生蚜酯酶(EST)活性及谷胱甘肽转移酶(GSH)活性的关系。结果表明,蚜虫EST活性与春小麦抗蚜水平有关,GSH活性与小麦抗蚜水平无关。穗部及旗叶蚜虫EST活性均与其存活率呈显著负相关,相关系数分别为-0.7047(P=0.04523)和-0.7214(P=0.0380),与有翅蚜分化率接近显著或显著负相关,相关系数分别为-0.6767(P=0.05911)和-0.7317(P=0.0336),与仔蚜繁殖率相关不显著。穗部及旗叶上蚜虫GSH活性均与其存活率、有翅蚜分化率及仔蚜繁殖率不相关。

Eight spring varieties with different degree of resistance to aphid（Sitobion avenae F.） were selected to study the esterase（EST） activity and glutathiones s-transferases（GSH） activity of female apterae after the artificial infestion during wheat grains filling stage.The result showed that both the survival rate of female apterae on panicle and on flag leaf negatively correlated to the aphids EST activity,with r=-0.7047（P=0.04523）and r=-0.7214（P=0.0380）,respectively,and the apterae alatae rate on panicle and on flag leaf also negatively correlated to the aphids EST activity,with r=-0.6767（P=0.05911）and r=-0.7317（P=0.0336）,respectively,but the progeny per female did not correlate to the aphids EST activity.Moreover,the GSH activity of female apterae did not correlate to the survival rate of female,apterae alatae rate and the Progeny per female.