测定维生素E的分光光度法研究

Study the Method of Spectrophotometry for Determination Vitamin E

本文研究并建立了定量分析维生素E的分光光度法。此方法基于维生素E结构中含有具有还原性的酚羟基,可定量与Fe^(3+)反应,还原为Fe^(2+),并与铁试剂α,α-联吡啶络合,络合物在520nm处有较大吸收,从而可以实现对维生素E的定量分析。该方法具有反应灵敏、设备简单、操作方便等特点,具有很广泛的适用性,方法的准确度以回收率表示为97.5％,精密度以变异系数表示为0.0072,最低检测浓度为7.15×10^(-7)mol/L(以α—V_E计算)。

The method of spectrophotometry for determination Vitamin E (VE) was developed. Using the reduction of phenolhydroxyl in VE the Fe3+ can be reduced Fe2+. When α, α' -dipyridine was adopted complex agent the red complex compound of Fe2+-α, α'-dipyridine was produced and absorbed at520nm on UV -VIS spectrometer.The method is simple and sensitive with detection limits 7.15+10~7mol/L (n = 5).