固定化对氯苯酚降解菌在生物流化床中的降解特性

Degradation characteristics of immobilized p-chlorophenol-degrading strain in bio-fluidized bed reactor

为了获得固定化对氯苯酚降解菌在生物流化床中的降解特性,用海藻酸钠-壳聚糖-活性炭微胶囊对实验室保存的对氯苯酚降解菌株进行固定化,在生物流化床中用固定化菌株在不同单因素实验条件下降解对氯苯酚废水,用气相色谱仪测定废水中对氯苯酚的降解率。结果表明,固定化对氯苯酚降解菌株处理污水的适宜条件为:HRT为72 h,微胶囊接种体积为废水体积的10%(其中微胶囊含菌量为3.0×106cfu/mL),降解pH值为7,降解温度为30℃,对氯苯酚初始浓度为120 mg/L。微胶囊固定化菌株与游离态菌株均可以降解对氯苯酚废水,但固定化菌株降解率优于游离态菌株,降解过程分别符合一级和二级动力学方程。

A p-chlorophenol-degrading strain was encapsulated in alginate-chitosan-activate carbon powder microcapsules,and the degradation characteristics of immobilized p-chlorophenol-degrading strain under different conditions in bio-fluidized bed reactor were investigated by gas chromtography.Results indicated that the optimum conditions for the degradation were HRT of 72 h,ratio of the volume of microencapsulated inoculation（about 3.0×106 cfu/mL in each microencapsulation） to the volume of wastewater of 10%,pH of 7,temperature of 30 ℃with the initial concentration of p-chlorophenol of 120 mg/L.Both of immobilized strain and free strain were capable of degrading p-chlorophenol and the kinetics was found to correlate with the primary and secondly kinetic equation respectively.But the degradation efficiency of the immobilized strain was higher than the free strain significantly.