姜黄素对大鼠心肌缺血再灌注损伤的保护作用

Protective effects of curcumin on myocardial ischemia reperfusion injury in rats

目的探讨姜黄素对大鼠心肌缺血再灌注引起的氧化应激损伤的保护作用。方法将SD大鼠随机分为6组,每组10只。(1)假手术组;(2)缺血再灌注损伤(MIR)组;(3)溶剂组;(4)姜黄素低、中、高剂量组。建立心肌缺血再灌注模型。用不同剂量姜黄素在结扎前30 min分别做预处理。再灌注结束后采血,测定丙二醛(MDA)、超氧化物歧化酶(SOD)、天门冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、乳酸脱氢酶同工酶(LDH1),并测量心肌梗死面积。结果姜黄素预处理能明显降低血浆中AST、LDH、LDH1和MDA的含量,提高SOD活性,减少心肌梗死面积。结论姜黄素对大鼠心肌缺血再灌注引起的氧化应激损伤有保护作用。

Objective To investigate the protective effect of curcumin on oxidative stress injury induced by myocardial ischemia reperfusion in rats.Methods Sixty healthy SD rats were randomized into six groups：sham group,MIR group,PEG group,MIR＋high dose curcumin treatment group,MIR＋middle dose curcumin treatment group,MIR＋low dose curcumin treatment group.Myocardial IR was carried out by ligation of left anterior descending coronary artery for 30 min followed by reperfusion for 60 min.Different dosages of curcumin were administered 30 min before the ligation.Blood was collected at the end of reperfusion for detecting changes of aspartate aminotransferase（AST）,lactate dehydrogenase（LDH）,lactate dehydrogenase1（LDH1）,superoxide dismutase（SOD）,and malondialdehyde（MDA）.The myocardial infarct size was also estimated.Results Curcumin pretreating dose-dependently decreased the contents of AST,LDH,LDH1 and MDA,and the myocardial infarct size,and increased the activity of SOD.Conclusion Curcumin exerts protective effects on myocardial ischemia reperfusion injury.