摘要
目的探讨依达拉奉抗谷氨酸诱导神经干细胞凋亡作用及发生机制。方法采用10μmol/L浓度的谷氨酸作用于大鼠13.5d胚胎皮层来源的神经干细胞,建立损伤模型。在建模同时采用依达拉奉进行干预,采用PI染色、Caspase-3染色及流式细胞仪检测。结果在依达拉奉干预下,依达拉奉治疗组凋亡细胞阳性率、Caspase-3阳性率等凋亡相关指标均明显降低,与谷氨酸组相比有统计学意义。结论依达拉奉可以拮抗谷氨酸诱导神经干细胞凋亡的效应,可能减低caspase-3,减少凋亡发生表达。
Objective To investigate the effect of edaravone of neural stem cells（NSCs） against glutamate.Methods NSCs were cultured from E 13.5D SD rat embryo.This experiment was subjected to 3 groups：control group,glutamate group with 10 nmol/L glutamate for culturing 24 hs,and edaravone group with 10 umol/L glutamate and edaravone 500 ng/ml together for culturing 24 hs.PI staining,caspase-3 staining and flow cytometry were used to analyze.Results Compared with those in glutamate group,the positive apoptosis rate and positive caspase-3 rate significantly decreased（P〈0.05）.Conclusion Edaravone can reduce apoptosis of NSCs following glutamate through reducing the expression of caspase-3.
出处
《海南医学》
CAS
2011年第2期23-24,共2页
Hainan Medical Journal
关键词
神经干细胞
凋亡
依达拉奉
Neural stem cells
Apoptosis
Edaravone
