VEGF对内皮抑素诱导的血管内皮细胞凋亡的抑制作用

The inhibitive effects of VEGF to apoptosis of human umbilical vein endothelial cells induced by recombinant adenovirus Ad-ES

目的探讨血管内皮生长因子(VEGF)对内皮抑素(ES)诱导的血管内皮细胞凋亡的抑制作用。方法构建携带ES基因的重组腺病毒Ad-ES感染脐静脉内皮细胞ECV-304,用RT-PCR及Western blot检测ES的转录和表达;实验组为Ad-ES+VEGF组,空白对照组为空载重组腺病毒Ad-Track组,阴性对照组为Ad-ES组,用流式细胞术及Hoechest 33258染色检测各组细胞凋亡情况。结果 Ad-ES感染ECV-304后检测到ES的转录和表达;Ad-ES组细胞发生凋亡,而Ad-Track组未观察到明显的细胞凋亡,Ad-ES+VEGF组细胞凋亡明显减少(P<0.05)。结论重组腺病毒Ad-ES在体外可诱导ECV-304细胞凋亡,这可能是ES抑制血管生成的重要机制;而VEGF可抑制这一作用。

Objective To evaluate the inhibitive effects of VEGF to apoptosis of human vascular endothelial cells induced by recombinant adenovirus Ad-ES.Methods The recombinant adenovirus was constructed with AdEasy-1 system and AAV293 cells.The transcription of ES and expression of ES protein was detected by RT-PCR and Western blot in ECV-304 cells infected with Ad-ES.The apoptosis were detected by flow cytometer and Hoechest 33258.Three groups were infected with Ad-ES＋VEGF,Ad-ES,Ad-Track respectively.Results The ES transcription and the expression were detected after infection.The apoptosis and growth inhibition of ECV-304 cells infected by Ad-ES and VEGF were decreased than those in the other groups.Conclusion Apoptosis of ECV-304 cells can be induced by Ad-ES,which may be one of the angiogenesis mechanism of ES.VEGF can inhibit this process.