小剂量VCR联合Endostar抑制PLA-802细胞系VEGF表达

The effect of the combination of small dosage of VCR and Endostar on the expression of VEGF in PLA-802 cell line

目的探讨小剂量长春新碱(VCR)联合恩度(Endostar,Endo)对横纹肌肉瘤细胞生长的影响及其机制。方法体外培养PLA-802细胞系,用小剂量Endo、VCR单独或联合处理细胞。用MTT和流式细胞仪检测细胞的生长和细胞周期;用RT-PCR和Western blot检测药物处理后细胞内VEGFmRNA和蛋白水平;用ELISA检测上清液中VEGF水平。结果单独应用VCR(0.5和2.0μg/mL)和单独应用Endo(2.0μg/mL)均能够抑制PLA-802细胞的增殖,并促进细胞的凋亡,抑制细胞内VEGF的表达和生成。而两者联合应用时,上述作用得到明显增强。结论小剂量VCR与Endo联合应用协同抑制PLA-802细胞中VEGF的生成和表达,诱导细胞凋亡。

Objective To explore the effect of the combination of small dosage of VCR and Endostar（Endo） on rhabdomyosarcoma cells and its mechanisms.Methods PLA-802 was cultured and treated with small dosage of VCR,Endostar singly or both.MTT was used to examine the cells proliferation,and flow cytometry was used for the checking cell cycle.While,RT-PCR and Western blot were for the expression of VEGF mRNA and protein levels.ELISA was for the generation of VEGF in supernatant.Results VCR（0.5 and 2.0 μg/mL） or Endo（2.0 μg/mL） used separcitely inhibited the cell proliferation and promoted the apoptosis,and also inhibited the expression and generation of VEGF.When the cells were treated with VCR and Endo together,the inhibitory effect of them were significantly strengthened.Conclusion The combination of VCR and Endostar synergistically inhibit the expression and generation of VEGF and induces the apoptosis in PLA-802 cells.