高压氧对实验兔海水型呼吸窘迫综合征的预防作用

Preventive effect of hyperbaric oxygen on seawater respiratory distress syndrome in experimental rabbits

目的 观察高压氧（ＨＢＯ）对海水淹溺肺水肿（ＰＥ－ＳＷＤ）的预防作用。方法 复制ＰＥ－ＳＷＤ动物模型后，３２ 只兔随机分为ＰＥ－ＳＷＤ对照组、药物治疗组和ＨＢＯ组。对３ 组兔的动脉血气酸碱指标、Ｃａ２＋ 沉淀反应颗粒、ｃ－ｆｏｓ ｍ ＲＮＡ和ｃ－ｊｕｎ ｍ ＲＮＡ 等进行自动检测和定量分析比较，并观察分析３组兔的存活时间和海水型呼吸窘迫综合征（ｓｅａｗ ａｔｅｒ－ｒｅｓｐｉｒａｔｏｒｙ ｄｉｓｔｒｅｓｓ ｓｙｎｄｒｏｍ ｅ，ＳＷ－ＲＤＳ）发生率。结果 ＨＢＯ组兔ＰａＯ２ ，ＳａＯ２ 和ｐＨ ３ 项指标比药物组显著升高（Ｐ＜ ０．０１），动物存活时间［（４３．０３±７．１９）小时］比药物组［（２３．５８±１．４９）小时］明显延长，Ｃａ２＋ 沉淀反应颗粒，ｃ－ｆｏｓ ｍ ＲＮＡ，ｃ－ｊｕｎ ｍ ＲＮＡ和ＳＷ－ＲＤＳ发生率ＨＢＯ组则明显低于药物组（Ｐ＜ ０．０１）。结论 ＨＢＯ可明显提高ＰａＯ２ 和ＳａＯ２，有效改善低氧血症和代谢性酸中毒，减轻ＰＥ－ＳＷＤ时肺组织的损伤，从而可防止ＰＥ－ＳＷＤ向ＳＷ－ＲＤＳ转化。

Objective To observe the effect of hyperbaric oxygen(HBO) on pulmonary edema after sea water drowning(PE SWD). Methods PE SWD was induced in all the 32 rabbits by infusing sea water into their lungs; then they were divided into the PE SWD control group, the medicinal group(MG) and the HBO group(HBO G). Blood gas and acid base indexes, Ca 2+ precipitating reaction products, c fos mRNA and c jun mRNA were measured and analyzed in all the three groups; at the same time, the survival time and incidence of seawater respiratory distress syndrome(SW RDS) were also observed. Results PaO 2,SaO 2 and pH increased remarkably( P <0 01) in HBO G. The survival time of rabbits in HBO G[(43 03±7 19)h]was longer than in MG[(23 58±1 49)h]; and the Ca 2+ precipitating reaction products,c fos mRNA,c jun mRNA and incidence of SW RDS were all significantly lower in HBO G. Conclusions HBO could obviously increase PaO 2 and SaO 2, improve effectively hypoxia and metabolic acidosis, and alleviate pulmonary tissue damage in PE SWD; therefore, the development of SW RDS might be prevented.