反相高效液相色谱法测定九圣散中苦杏仁苷的含量

Determination of Amygdalin in Jiusheng Powder by RP-HPLC

目的:建立测定九圣散中苦杏仁苷含量的方法。方法:采用反相高效液相色谱法,色谱条件为ZORBAX SB-C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸溶液(6∶94),流速为0.8 mL/min,柱温:30℃,检测波长为207 nm。结果:苦杏仁苷在6.0-120.0 g/L(r=0.9998)浓度范围内有良好的线性关系,平均加样回收率为99.7%,RSD为1.20%。结论:反相高效液相色谱法简便,快速,准确,重复性好,可用于九圣散的质量控制。

Objective： To establish a method for the determination of amygdalin in Jiusheng powder.Methods： The RP-HPLC system consisting the ZORBAX SB-C18（4.6 mm×250 mm,5 μm）column,the mobile phase consisted of Acetonitrile-0.1% H3PO4（6∶94）,the flow rate was 0.8 mL/min,the column temperature was 30℃,The UV detector was set at 207 nm.Results： The linear response range was 6.0-120.0 μg/mL（r=0.9998）.The average recovery of amygdalin was 99.7%,RSD was 1.20%.Conclusion： The assay demonstrated that the method had adequate and selectivily to measure the concentrations of simple and accurate,repeatable,it can be applied in quantitative determination of amygdalin in Jiusheng powder.