肾癌细胞c-myc的表达分布及其临床意义

Cellular localization of c-myc product and clinical significance in renal cell carcinoma

目的：阐明ｃｍ ｙｃ 表达在肾癌发生、发展中的作用及临床意义。方法：利用免疫组织化学 Ａ Ｂ Ｃ 法结合微波抗原修复和流式细胞技术，对ｃｍ ｙｃ 蛋白在 ５２ 例肾癌组织及 １４ 例癌旁正常肾组织中的表达进行了定性、定量的测定。结果：肾癌组织中ｃｍ ｙｃ 蛋白的阳性率和阳性细胞率分别为７６．９２％ 和６５．４５％ ±２９．１９％ ，高于正常肾组织的１４．２９％ 和８．５９％ ±３．６６％ （ Ｐ ＜ ０．００５）。ｃｍ ｙｃ在肾癌组织中５５．７７％ 分布于胞浆，２１．１５％ 分布于胞核，而在正常肾组织则全部分布于胞核。其胞浆表达在病理 Ｇ２、 Ｇ３ 级高于 Ｇ１ 级（χ２＝ ９．２４４， Ｐ ＜ ０．００５）；临床分期 Ｔ３、 Ｔ４ 期高于 Ｔ１、 Ｔ２ 期（ Ｐ＜ ０．００１）。结论：ｃｍ ｙｃ 的胞浆表达促进肾癌的发生、发展，并与恶性程度、临床分期有关，是预后不良的重要指征。

Purpose:To get to know the effect of c myc protein on the onset and development of renal cell carcinoma and clinical progress.Methods:We have detected expression of c myc protein qualitatively and quantitatively in 14 cases normal tissue and 52 cases renal cell carcinoma(RCC). Using immunohistochemical technique,and cytoflowmetry and cytoimmunofluorescence.Results:Normal tissue showed staining only of some nuclei while in RCC staining varied from predominantly nuclear to focal or pancytoplasma. Positive rate and positive cell rate of c myc protein were 76.92 % and 65.45 %± 29.19 % respectively in RCC,they were significant higher than those ( 14.29 % and 8.59 %± 3.66 %) of the normal tissue (P< 0.005 ). Cytoplasmic expression of c myc was related to high pathological grade (χ 2= 9.244 ,P< 0.005 ) and high clinical stage (P< 0.001 ).Conclusions:Cytoplasmic expression of c myc might play important roles in the pathogenisis and was helpful in the evaluation of prognose of patients in RCC.