黄竹清脑颗粒对脑出血急性期(痰热腑实证)大鼠脑组织细胞凋亡影响的实验研究

Effect of Huangzhu Qingnao Granule on The Brain Tissue Apoptosis in Acute Intracerebral Hemorrhage (Tanre Fushi syndrome) Rats

目的:研究黄竹清脑颗粒对脑出血急性期(痰热腑实证)大鼠血肿周围脑组织细胞凋亡的影响。方法:采用胶原酶Ⅶ-肝素混合液立体定位注射及配合SD大鼠自体粪便灌胃的方法制作60只脑出血急性期(痰热腑实证)大鼠模型,将大鼠随机分为空白对照组、痰热腑实证模型组、黄竹清脑颗粒大、中、小剂量组及清开灵口服液组,采用TUNEL法(脱氧核苷酸末端转移酶介导的缺口末端标记法)进行脑组织细胞凋亡的检测。结果:(1)痰热腑实证模型组与空白对照组对比,前者大鼠血肿周围脑组织细胞凋亡阳性率明显高于后者大鼠正常脑组织(P<0.01),提示造模成功;(2)黄竹清脑颗粒大、中剂量组疗效优于清开灵口服液组及黄竹清脑颗粒小剂量组(P<0.05);结论:黄竹清脑颗粒能明显减轻脑出血期(痰热腑实证)大鼠粪便干结、烦躁易动、口干多饮、喉间痰鸣、舌苔黄腻等症状,改善大鼠脑出血后神经系统病态体征,并具有明显降低大鼠脑组织细胞凋亡,起到保护脑细胞作用。

Objective：To study the Huangzhu Qingnao granule on acute intracerebral hemorrhage（Tanre Fushi syndrome） in rat brain tissue surrounding hematoma apoptosis. Methods：Collagenase Ⅶ-heparin mixture of stereotactic injection and with the SD rats were fed with autologous method of production of faeces rats（60） with acute intracerebral hemorrhage（Tanre Fushi syndrome） rat model,rats were randomly divided into blank control group,Tanre Fushi syndrome model group,Huangzhu Qingnao granules large,medium,low-dose group and Qingkailing oral group,using TUNEL method（terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling） in the brain cell apoptosis detection. Results：（1） Tanre Fushi syndrome model group compared with the blank control group,the former in rat brain tissue surrounding hematoma positive rate of apoptosis,which was significantly higher than normal brain tissue in rats（P 0. 01） ,prompted modeling success; （2） Huangzhu Qingnao granule of large,medium-dose group were superior efficacy of oral liquid group and Qingkai ling Huangzhu Qingnao granule is small-dose group（P 0. 05） . Conclusion：Huangzhu Qingnao granule particles could significantly reduce the cerebral hemorrhage period（Tanre Fushi syndrome） Rat droppings dry,irritability easy to move,dry mouth,polydipsia,throat Phlegm-ming,greasy yellow tongue and other symptoms and improve the nervous system in rats after intracerebral hemorrhage in pathological signs,and has significantly reduced apoptosis in rat brain cells,play a role in protecting brain cells.