UPLC法测定莲菊感冒胶囊中3种成分的含量

Simultaneous determination of 3 components in Lianju Ganmao capsule by UPLC

目的：建立同时测定莲菊感冒胶囊中1,5-O-二咖啡酰基-奎宁酸、3,5-O-二咖啡酰基-奎宁酸、3,4-O-二咖啡酰基-奎宁酸的超高效液相色谱（UPLC）分析方法。方法：采用Waters Acquity UPLC系统,Ac-quity UPLC BEHC18色谱柱（100 mm×2.1mm,1.7μm）,流动相为乙腈-0.1%磷酸,以流速为0.2 mL.min-1进行梯度洗脱;柱温：45℃;样品经50%乙醇超声提取后用UPLC-PDA进行分析检测,检测波长为325 nm。结果：1,5-O-二咖啡酰基奎宁酸、3,5-O-二咖啡酰基奎宁酸、3,4-O-二咖啡酰基奎宁酸3种被测成分在线性范围内均具有良好的线性关系（r≥0.999 8）;平均回收率在99.1%~101.7%之间,RSD≤2.5%。结论：UPLC分离效果及重复性好,且快速、简便,可作为莲菊感冒胶囊的质量控制方法。

Objective： To establish an ultra performance liquid chromatography（UPLC） method for simultaneous determination of 3 components in Lianju Ganmo capsule.Methods： The analyses were performed on Waters Acquity UPLC system.An Acquity UPLC BEH C18 column（100 mm×2.1 mm,1.7 μm） was used for all analyses.The investigated compounds were separated with gradient mobile phase consisting of 0.1% phosphoric acid and acetonitrile.The flow velocity was 0.2 mL·min-1.The temperature of sample manager was set at 45℃.UPLC-PDA system was used to analysis the sample which the sample mix in 50% ethanol through ultrasound.The detection wavelength was 325 nm.Results： The investigated compounds including 1,5-O-dicaffeoyl-quinic acid 3,5-O-dicaffeoyl-quinic acid 3,4-O-dicaffeoyl-quinic acid,had a good linearity（r≥0.999 8） over the tested ranges.The average recovery was 99.1% to 101.7% with RSD≤2.5%.Conclusion： The developed UPLC method is simple,sensitive and accurate with good repeatability,which is available for quality control of Lianju Ganmao capsule.