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细胞外钙离子对人成骨样细胞MG-63分化的影响 被引量：3

Effect of extracellular Ca^(2+) on osteoblast differentiation in MG-63 cells
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摘要 研究不同浓度钙离子对人成骨样细胞MG-63分化的影响.结果表明,5.0mmol/LCa2+促进CaSR、ALP、OC、Runx2、IGF-1和BMP2 mRNA表达,而加入CaSR和ERK抑制剂后,则下调ALP、OC、Runx2、IGF-1和BMP2 mRNA表达.不同浓度Ca2+处理后,磷酸化-ERK(p-ERK)蛋白表达明显增加.因此,5.0mmol/L Ca2+通过上调CaSR基因的表达,激活ERK通路,促进MG-63成骨样细胞的分化. In the present study we have examined effects of extracellular Ca2＋ on osteoblast differentiation in MG-63 cells.The results showed that 5.0mmol/L Ca2＋ up-regulated the expression of CaSR,OC、ALP、Runx2、 IGF-1,and BMP2.However,these indicators decreased after treatment with blocking agents.In addition,5.0 and 10.0mmol/L Ca2＋ increased phospho-ERK expression,but did not affect ERK.Therefore 5.0mmol/L Ca2＋ up-regulated CaSR and then promoted osteoblast differentiation through ERK pathway in MG-63 cells.
作者 郑家强 杨建虹
机构地区 中国科学院研究生院
出处 《中国科学院研究生院学报》 CAS CSCD 北大核心 2011年第1期57-64,共8页 Journal of the Graduate School of the Chinese Academy of Sciences
基金 中国科学院研究生院启动基金(055101FM03)资助
关键词 钙离子 分化 成骨细胞 CASR ERK Ca2＋ differentiation osteoblast CaSR ERK
分类号 Q2 [生物学—细胞生物学]
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共引文献28

同被引文献43

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中国科学院研究生院学报
2011年 第1期

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