MSTN基因外显子3的多态性及其与边鸡生长性状的关联分析

Polymorphisms in Exon 3 of the Myostatin Gene and its Relationship with Growth Traits in Bian Chicken

采用PCR-SSCP技术检测边鸡及2个对照鸡群体（京海黄鸡和尤溪麻鸡）肌肉生长抑制素（myostatin,MSTN）基因外显子3的单核苷酸多态性,并与边鸡的生长性状进行相关性分析。结果表明,在MSTN基因外显子3的8527—8528 bp处检测到一个TC的插入。在边鸡和京海黄鸡中只检测到2种基因型（AA和AB基因型）,在尤溪麻鸡中检测到3种基因型（AA、AB和BB基因型）。在3个鸡品种中都以A等位基因为优势等位基因。最小二乘分析结果表明,边鸡2种基因型个体的6~16周龄体重均无显著差异（P〉0.05）。

Single nucleotide polymorphisms（SNPs） of exon 3 of the myostatin（MSTN） gene were detected in the Bian chicken and two reference chicken populations（Jinghai and Youxi chickens） by PCR-SSCP.The relationship with growth traits in the Bian chicken was also analysed.Results showed that there exsited an insretion（TC） in the 8527 to 8528 bp of the exon 3 of the MSTN gene.Three genotypes（AB,AB and BB） were detected in the Youxi chicken,while the Bian and Jinghai chickens only displayed two genotypes（AA and AB）.The allele A was the predominant allele in the three chicken breeds.The least square means analysis showed the differences of body weight between the two genotypes in the Bian chicken were nonsignificant（P0.05）.