中国西门塔尔牛GH基因SNPs与经济性状的关联分析

Association analysis of SNPs of GH Gene and Economic Traits of Chinese Simmental Populations

本试验采用PCR-RFLP和DNA测序等技术检测牛生长激素基因P3位点(growth hormone,GH-P3)在中国西门塔尔牛群体中的多态性,并利用最小二乘法拟合线性模型对该位点与牛经济性状进行关联分析。结果表明,BB基因型个体胴体重和净肉重极显著高于AA和AB基因型个体(P<0.01),屠宰率和净肉率显著高于AA和AB基因型(P<0.05),AA基因型背膘厚极显著低于AB、BB基因型(P<0.01)。本研究结果显示,GH-P3位点对中国西门塔尔牛个体胴体重、屠宰率、净肉重、净肉率及背膘厚等经济性状有显著或极显著的影响,为中国西门塔尔牛重要经济性状的分子标记辅助选择(MAS)及品系培育提供理论依据。

Based on the technologies of PCR-RFLP and DNA sequencing,the polymorphism of GH-P3 position within growth hormone（bGH） gene among the individuals of Chinese Simental population was estimated,and associated with the economic traits of Simmental populations by using the least square meas（LSM） based general lineal model（GLM）.The results showed that individuals with BB genotype possessed statistically heavier carcass weight than those with AA and AB genotype（P0.01）,the dressing percentage and meat percentage of BB genotype individuals were higher than AA and AB genotypes respectively（P0.05）,and the backfat thickness of AA genotype was lower than AB and BB genotypes（P0.01）.These results indicated that position of GH-P3 within bGH gene significanlty and very significanlty influences the economic traits including carcass weight,dressing percentage,carcass weight,meat percentage and backfat thickness of Chinese Simmental population,which provided theoretical references for the molecular assisted selection（MAS） of key economic traits and germplasm enhancement of Chinese Simmental populations.