人颗粒细胞共培养对牛卵母细胞体外成熟及孤雌激活的影响

Effect of Co-culturing with Human Granule Cells on the IVM and Parthenogenetic Activation of Bovine Oocytes

为了探讨人颗粒细胞共培养对牛卵母细胞体外成熟及孤雌激活的影响,将穿刺获得的牛卵母细胞随机分为试验组和对照组,试验组以人颗粒细胞作为滋养层与牛卵母细胞在体外成熟液中共培养,对照组仅使用与试验组相同的体外成熟液培养,观察并比较两组的极体排出率、孤雌激活囊胚形成率。结果发现,试验组的极体排出率(72.35%)显著高于对照组的极体排出率(52.86%)(P<0.01);经过孤雌激活后,试验组的囊胚形成率为12.82%,对照组为4.76%,两组之间无显著性差异(P>0.05)。说明人颗粒细胞可以作为牛卵母细胞体外成熟时的滋养层,两者共培养可以提高牛卵母细胞体外培养的核成熟率。

In order to evaluate the effect of co-culturing with human granule cells on the IVM of bovine oocytes,we divided the bovine oocytes into the experimental group and the control group.In the experimental group,bovine oocytes were co-culturing with human granule cells in the IVM medium.While in the control group bovine oocytes were cultured in the same IVM medium.The rates of maturating and blastocyst formation rates through parthenogenetic activation were compared between the two groups.The maturating rates in the experimental group（72.35%） was higher than that in the control group（52.86%）（P0.01）.After parthenogenetic activation,the blastocyst formation rates in the two groups were 12.82% and 4.76%,but the differences were not significant（P0.05）.The results suggested that human granule cells can be used for co-culturing in the IVM of bovine oocytes,co-culturing with human granule cells may elevate the nuclear maturing rates.