抑制性消减杂交和基因芯片技术在牙髓差异表达基因研究中的应用

Technologies of suppression subtractive hybridization and DNA chip technique and their applications in dental pulp studies

基因表达的时、空、量模式的变化引起了生命过程中细胞的发育和分化、细胞周期的调节、细胞的衰老和死亡以及细胞的病理过程。寻找差异表达基因成为目前基因研究的一个非常重要的内容。近年来,分离差异表达基因的技术不断完善,先后出现了消减杂交、mRNA差异显示法、代表性差异分析法、基因表达系列分析、抑制性消减杂交和(半)定量聚合酶链反应等多种分析技术。本文就牙髓研究中的2种常用的大规模快速筛选差异基因的分析方法和应用进展作一综述。

Temporal and spatial distinct patterns of gene expression regulate biological processes involved in cell development,differentiation,cell cycle,death and pathology.The identification of differential expression gene between the different functional status cells has become an important content of gene research.In recent years,a variety of methods have been developed for analying differentially expressed genes,including subtractive hybridization,mRNA differential display,representational difference analysis,serial analysis of gene expression,suppression subtractive hybridization,expressed sequence tags,DNA chip,quantitative polymerase chain reaction and semiquantitative polymerase chain reaction.This manuscript reviews the two most commonly used technologies of rapid and large-scale screening for differentially expressed genes,and highlights recent applications in dental pulp studies.